Detection of Feline Leukemia virus p27 antigen by microELISA vs immunochromatographic tests in cats from Guayaquil
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Abstract
The domestic cat is one of the pets of choice, which leads to the evaluation of diagnostic methods for diseases such as feline viral leukemia or Vilef, where the efficacy and speed of diagnosis is important because it is a highly fatal disease. The aim of this study was to determine the percentage of sensitivity of two immunochromatography tests commonly used in veterinary offices to diagnose Vilef and to check their usefulness by making a comparison with the results of the microELISA method, as a reference technique. Using a quantitative, experimental, applied approach, the research was developed from the collection of blood sera from cats in a clinical laboratory in the city of Guayaquil, Ecuador. Forty blood sera were analyzed for detection of feline leukemia virus p27 antigen by the microELISA method and later with immunochromatography tests. The results determined that when reaching a kappa value range of 0.8, the tests present a very good concordance degree, resulting in a 93 % of sensitivity. Concluding that the tests evaluated are reliable and can be considered as tools for rapid diagnosis.
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